Pluripotent stem cell differentiation pathway (WP2848)

This pathway provides an overview of the directed differentiation molecules used to induce early and derivative cell lineages from human pluripotent stem cells. This overview differentiates between the three primary germ cell layers (ectoderm-outer layer, endoderm-inner layer, mesoderm-middle layer), which are formed in the earliest state of embryonic development and give rise to different tissue types. The initial version of this pathway is a direct adaptation of the SnapShot "Directed Differentiation of Pluripotent Stem Cells" pathway authored by Luis A. Williams, Brandi N. Davis-Dusenbery, and Kevin C. Eggan, HHMI, Harvard University, Cell 149, May 25, 2012 Elsevier Inc. DOI 10.1016/j.cell.2012.05.015. http://download.cell.com/pdf/PIIS0092867412005946.pdf. This adaptation was generated by Meenakshi Venkatasubramanian and Krithika Ramasamy Subramanian at Cincinnati Children's Hospital in the laboratory of Nathan Salomonis. Proteins on this pathway have targeted assays available via the [https://assays.cancer.gov/available_assays?wp_id=WP2848 CPTAC Assay Portal]
last edited

Authors

Nsalomonis, Khanspers, AlexanderPico, Egonw, MaintBot, Zari, Susan, DeSl, Ddigles, Fehrhart, and Eweitz

Cited In

Organism

Homo sapiens

Communities

Annotations

Disease Ontology: post-traumatic stress disorder spinal cord disease

Cell Type Ontology: astrocyte of the spinal cord stem cell

Pathway Ontology: regulatory pathway growth factor signaling pathway

Participants

Label Type Compact Identifier
Ascorbic acid Metabolite hmdb:HMDB0000044
NT5E GeneProduct ensembl:ENSG00000135318
FGF2 GeneProduct ensembl:ENSG00000138685
A-83-01 Metabolite pubchem.compound:16218924
Selenium Metabolite hmdb:HMDB0001349
CXCR1 GeneProduct ensembl:ENSG00000163464
RA Metabolite hmdb:HMDB0001852
SHH GeneProduct ensembl:ENSG00000164690
Niacinamide Metabolite hmdb:HMDB0001406
BMP4 GeneProduct ensembl:ENSG00000125378
TGFB1 GeneProduct ncbigene:7040
NOG GeneProduct ensembl:ENSG00000183691
BMP4 GeneProduct ensembl:ENSG00000125378
SB431542 Metabolite pubchem.compound:4521392
Cyclopamine Metabolite pubchem.compound:442972
DAPT Metabolite pubchem.compound:5311272
KIT GeneProduct ensembl:ENSG00000157404
EGF GeneProduct ensembl:ENSG00000138798
VEGFA GeneProduct ensembl:ENSG00000112715
FGF4 GeneProduct ensembl:ENSG00000075388
IGF1 GeneProduct ensembl:ENSG00000017427
HGF GeneProduct ensembl:ENSG00000019991
NOTCH1 GeneProduct ncbigene:4851
EGF GeneProduct ensembl:ENSG00000138798
BMP4 GeneProduct ensembl:ENSG00000125378
NOG GeneProduct ensembl:ENSG00000183691
WNT3A GeneProduct ensembl:ENSG00000154342
HGF GeneProduct ensembl:ENSG00000019991
FGF4 GeneProduct ensembl:ENSG00000075388
EGF GeneProduct ensembl:ENSG00000138798
NODAL GeneProduct ensembl:ENSG00000156574
FGF10 GeneProduct ensembl:ENSG00000070193
RA Metabolite hmdb:HMDB0001852
FGF10 GeneProduct ensembl:ENSG00000070193
BMP4 GeneProduct ensembl:ENSG00000125378
VEGFA GeneProduct ensembl:ENSG00000112715
SCF GeneProduct ensembl:ENSG00000049130
TPO GeneProduct ensembl:ENSG00000115705
FLT3LG GeneProduct ensembl:ENSG00000090554
SCF GeneProduct ensembl:ENSG00000049130
CSF1 GeneProduct ensembl:ENSG00000184371
IL6 GeneProduct ensembl:ENSG00000136244
IL3 GeneProduct ensembl:ENSG00000164399
VEGFA GeneProduct ensembl:ENSG00000112715
BMP4 GeneProduct ensembl:ENSG00000125378
SCF GeneProduct ensembl:ENSG00000049130
TPO GeneProduct ensembl:ENSG00000115705
IL3 GeneProduct ensembl:ENSG00000164399
EPO GeneProduct ensembl:ENSG00000130427
INHBA GeneProduct ensembl:ENSG00000122641
FGF2 GeneProduct ensembl:ENSG00000138685
BMP4 GeneProduct ensembl:ENSG00000125378
FLT3LG GeneProduct ensembl:ENSG00000090554
TPO GeneProduct ensembl:ENSG00000115705
FGF2 GeneProduct ensembl:ENSG00000138685
VEGFA GeneProduct ensembl:ENSG00000112715
FGF2 GeneProduct ensembl:ENSG00000138685
SB431542 Metabolite pubchem.compound:4521392
TGFB1 GeneProduct ncbigene:7040
PDGFB GeneProduct ensembl:ENSG00000100311
IL3 GeneProduct ensembl:ENSG00000164399
IL6 GeneProduct ensembl:ENSG00000136244
VEGFA GeneProduct ensembl:ENSG00000112715
SCF GeneProduct ensembl:ENSG00000049130
IL11 GeneProduct ensembl:ENSG00000095752
DKK1 GeneProduct ensembl:ENSG00000107984
VEGFA GeneProduct ensembl:ENSG00000112715
WNT1 GeneProduct ensembl:ENSG00000125084
DKK1 GeneProduct ensembl:ENSG00000107984
WNT1 GeneProduct ensembl:ENSG00000125084
VEGFA GeneProduct ensembl:ENSG00000112715
TGFB3 GeneProduct ensembl:ENSG00000119699
NTF4 GeneProduct ensembl:ENSG00000225950
FGF2 GeneProduct ensembl:ENSG00000138685
BMP4 GeneProduct ensembl:ENSG00000125378
FST GeneProduct ensembl:ENSG00000134363
NTF4 GeneProduct ensembl:ENSG00000225950
BMP4 GeneProduct ensembl:ENSG00000125378
FGF2 GeneProduct ensembl:ENSG00000138685
GDF5 GeneProduct ensembl:ENSG00000125965
Ascorbic acid Metabolite hmdb:HMDB0000044
Beta-Glycerophosphoric acid Metabolite hmdb:HMDB0002520
Dexamethasone Metabolite hmdb:HMDB0015364
Dexamethasone Metabolite hmdb:HMDB0015364
INS GeneProduct ensembl:ENSG00000254647
INS GeneProduct ensembl:ENSG00000254647
TF GeneProduct ensembl:ENSG00000091513
VEGFA GeneProduct ensembl:ENSG00000112715
TPO GeneProduct ensembl:ENSG00000115705
EPO GeneProduct ensembl:ENSG00000130427
IL6 GeneProduct ensembl:ENSG00000136244
IL3 GeneProduct ensembl:ENSG00000164399
IL11 GeneProduct ensembl:ENSG00000095752
CSF1R GeneProduct ensembl:ENSG00000182578
TNFSF11 GeneProduct ensembl:ENSG00000120659
BMP4 GeneProduct ensembl:ENSG00000125378
WNT2B GeneProduct ncbigene:7482
WNT5A GeneProduct ncbigene:7474
WNT7B GeneProduct ncbigene:7477
WNT2 GeneProduct ncbigene:7472
FGF2 GeneProduct ensembl:ENSG00000138685
IL6 GeneProduct ensembl:ENSG00000136244
IL6R GeneProduct ensembl:ENSG00000160712
BMP4 GeneProduct ensembl:ENSG00000125378
FGF2 GeneProduct ensembl:ENSG00000138685
INHBA GeneProduct ensembl:ENSG00000122641
SHH GeneProduct ensembl:ENSG00000164690
Niacinamide Metabolite hmdb:HMDB0001406
Taurine Metabolite hmdb:HMDB0000251
IGF1 GeneProduct ensembl:ENSG00000017427
FGF8 GeneProduct ensembl:ENSG00000107831
WNT1 GeneProduct ensembl:ENSG00000125084
ALK GeneProduct ensembl:ENSG00000171094
Vitamin A Metabolite wikidata:Q424976
LEFTY1 GeneProduct ensembl:ENSG00000243709
SHH GeneProduct ensembl:ENSG00000164690
BMP4 GeneProduct ensembl:ENSG00000125378
CNTF GeneProduct ensembl:ENSG00000242689
Naltrexone Metabolite hmdb:HMDB0014842
FGF2 GeneProduct ensembl:ENSG00000138685
NODAL GeneProduct ensembl:ENSG00000156574
DKK1 GeneProduct ensembl:ENSG00000107984
PDGFA GeneProduct ensembl:ENSG00000197461
Retinoic acid Metabolite hmdb:HMDB0001852
EGF GeneProduct ensembl:ENSG00000138798
FGF1 GeneProduct ensembl:ENSG00000113578
FGF2 GeneProduct ensembl:ENSG00000138685
NOG GeneProduct ensembl:ENSG00000183691
Vitamin A Metabolite wikidata:Q424976
SHH GeneProduct ensembl:ENSG00000164690
SHH GeneProduct ensembl:ENSG00000164690
INHBA GeneProduct ncbigene:3624
INHBA GeneProduct ncbigene:3624
BMP4 GeneProduct ncbigene:652
IGF1 GeneProduct ensembl:ENSG00000017427
TPO GeneProduct ensembl:ENSG00000115705
EPO GeneProduct ensembl:ENSG00000130427
FLT3LG GeneProduct ensembl:ENSG00000090554
FGF2 GeneProduct ensembl:ENSG00000138685

References

  1. David S, Kroner A. Repertoire of microglial and macrophage responses after spinal cord injury. Nat Rev Neurosci. 2011 Jun 15;12(7):388–99. PubMed Europe PMC Scholia
  2. Poulain M, Ober EA. Interplay between Wnt2 and Wnt2bb controls multiple steps of early foregut-derived organ development. Development. 2011 Aug;138(16):3557–68. PubMed Europe PMC Scholia
  3. Chou ST, Byrska-Bishop M, Tober JM, Yao Y, Vandorn D, Opalinska JB, et al. Trisomy 21-associated defects in human primitive hematopoiesis revealed through induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17573–8. PubMed Europe PMC Scholia