One-carbon metabolism (WP435)

This one-carbon metabolism pathway is centered around folate. Folate is the primary methyl-group donor for processes such as DNA methylation reactions, nucleotide synthesis and DNA repair mechanisms. An important pathway for any study related to folate or DNA methylation. Differential methylation (e.g. hypermethylation of tumor suppressors) as well as disturbances in nucleotide synthesis and repair, are associated with several forms of cancer. There are also indications that hypermethylation is involved in the progression of adenomas to cancer. Inferred from human one-carbon metabolism pathway.
last edited


Michiel, Khanspers, MaintBot, AlexanderPico, Evelo, Ddigles, Mkutmon, Egonw, Fehrhart, and Eweitz

Cited In


Mus musculus




Pathway Ontology: folate mediated one-carbon metabolic pathway


Label Type Compact Identifier
Mthfd1 GeneProduct ncbigene:108156
Tetrahydrofolate Metabolite cas:135-16-0
Mthfd1 GeneProduct ncbigene:108156
L-Methionine Metabolite hmdb:HMDB0000696
Mtfmt GeneProduct ncbigene:69606
Dnmt1 GeneProduct ncbigene:13433
Gart GeneProduct ncbigene:14450
Dnmt3b GeneProduct ncbigene:13436
Dhfr GeneProduct ncbigene:13361
Matia GeneProduct ncbigene:11720
Bhmt GeneProduct ncbigene:12116
5-Formiminotetrahydrofolic acid Metabolite hmdb:HMDB0001534
Mthfd1 GeneProduct ncbigene:108156
Mat2b GeneProduct ncbigene:108645
Atic GeneProduct ncbigene:108147
Ftcd GeneProduct ncbigene:14317
5-Formyltetrahydrofolate Metabolite hmdb:HMDB0001562
Tcn II GeneProduct ncbigene:21452
Mthfd2 GeneProduct ncbigene:17768
5,10-Methenyl Tetrahydrofolate Metabolite hmdb:HMDB0001354
Amt GeneProduct ncbigene:434437
Dihydrofolate Metabolite cas:4033-27-6
Monoglutamate Metabolite cas:59-30-3
Glycine Metabolite hmdb:HMDB0000123
Shmt2 GeneProduct ncbigene:108037
Mtr GeneProduct ncbigene:238505
Amt GeneProduct ncbigene:434437
Folh1 GeneProduct ncbigene:53320
Ehmt1 GeneProduct ncbigene:77683
Ahcy GeneProduct ncbigene:269378
S-Adenosylhomocysteine Metabolite hmdb:HMDB0000939
Shmt1 GeneProduct ncbigene:20425
Polyglutamate Metabolite cas:25513-46-6
5-Methyl Tetrahydrofolate Metabolite cas:134-35-0
Riboflavin/Vitamin B2 Metabolite hmdb:HMDB0000244
Mthfd1l GeneProduct ncbigene:270685
Homocysteine Metabolite hmdb:HMDB0000742
Mthfd2 GeneProduct ncbigene:17768
dUMP Metabolite chebi:17622
Dnmt3a GeneProduct ncbigene:13435
Mthfr GeneProduct ncbigene:17769
Methylcobalamin Metabolite hmdb:HMDB0002274
Mtrr GeneProduct ncbigene:210009
Ehmt2 GeneProduct ncbigene:110147
L-Serine Metabolite hmdb:HMDB0000187
Mthfd1l GeneProduct ncbigene:270685
Ftcd GeneProduct ncbigene:14317
Mthfd1l GeneProduct ncbigene:270685
dTMP Metabolite chebi:17013
Ethanol Metabolite hmdb:HMDB0000108
S-Adenosylmethionine Metabolite chebi:15414
Cobalamin Metabolite hmdb:HMDB0002174
Aldh1l1 GeneProduct ncbigene:107747
Kiaa0828 GeneProduct ncbigene:74340
10-Formyl Tetrahydrofolate Metabolite cas:2800-34-2
Gart GeneProduct ncbigene:14450
Dhfr GeneProduct ncbigene:13361
Tyms GeneProduct ncbigene:22171
Betaine Metabolite hmdb:HMDB0000043
Dhfr GeneProduct ncbigene:13361
Mthfs GeneProduct ncbigene:107885
5,10-Methylene Tetrahydrofolate Metabolite chebi:12071


  1. Hazra A, Wu K, Kraft P, Fuchs CS, Giovannucci EL, Hunter DJ. Twenty-four non-synonymous polymorphisms in the one-carbon metabolic pathway and risk of colorectal adenoma in the Nurses’ Health Study. Carcinogenesis. 2007 Jul;28(7):1510–9. PubMed Europe PMC Scholia