mRNA processing (WP529)

This process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' polyadenylation, and RNA splicing, which occur in the cell nucleus before the RNA is translated. 5' Capping: Capping of the pre-mRNA involves the addition of 7-methylguanosine (m7G) to the 5' end. The cap protects the 5' end of the primary RNA transcript from attack by ribonucleases that have specificity to the 3'5' phosphodiester bonds. 3' Processing: The pre-mRNA processing at the 3' end of the RNA molecule involves cleavage of its 3' end and then the addition of about 200 adenine residues to form a poly(A) tail. As the poly(A) tails is synthesised, it binds multiple copies of poly(A) binding protein, which protects the 3'end from ribonuclease digestion. Splicing: RNA splicing is the process by which introns, regions of RNA that do not code for protein, are removed from the pre-mRNA and the remaining exons connected to re-form a single continuous molecule. Description adapted from Wikipedia: http://en.wikipedia.org/wiki/Post-transcriptional_modification Pathway adapted from http://www.reactome.org.
last edited

Authors

Nsalomonis, MaintBot, Khanspers, Ddigles, Mkutmon, and Eweitz

Cited In

Organism

Rattus norvegicus

Communities

Annotations

Pathway Ontology: transcription pathway spliceosome pathway

Participants

Label Type Compact Identifier
Prpf4 GeneProduct ensembl:ENSRNOG00000014777
U4 snRNA Rna None
Snrpa1 GeneProduct ensembl:ENSRNOG00000011932
Rbm17 GeneProduct ensembl:ENSRNOG00000018767
Snrpd3 GeneProduct ensembl:ENSRNOG00000050410
Ncbp2 GeneProduct ensembl:ENSRNOG00000001746
DNAJC8 GeneProduct None
Rngtt GeneProduct ensembl:ENSRNOG00000007867
Snrpd1 GeneProduct ensembl:ENSRNOG00000013714
Sf3a2 GeneProduct ensembl:ENSRNOG00000019349
SNRPD2 GeneProduct None
Xrn2 GeneProduct ensembl:ENSRNOG00000011785
Rnu6 Rna None
Dhx16 GeneProduct ensembl:ENSRNOG00000030157
Hnrnpm GeneProduct ensembl:ENSRNOG00000007921
AABR07060628.1 GeneProduct ensembl:ENSRNOG00000051748
Celf2 GeneProduct ensembl:ENSRNOG00000023661
Spop GeneProduct ensembl:ENSRNOG00000004686
Hnrnpa2b1 GeneProduct ensembl:ENSRNOG00000011175
Phf5a GeneProduct ensembl:ENSRNOG00000024170
Sf3b5 GeneProduct ensembl:ENSRNOG00000014908
Hnrnpr GeneProduct ensembl:ENSRNOG00000011910
Prpf6 GeneProduct ensembl:ENSRNOG00000051498
Ybx1 GeneProduct ensembl:ENSRNOG00000023786
Srsf9 GeneProduct ensembl:ENSRNOG00000001163
Sugp1 GeneProduct ensembl:ENSRNOG00000052111
Snu13 GeneProduct ensembl:ENSRNOG00000025154
Sf3b3 GeneProduct ensembl:ENSRNOG00000017724
Prpf40a GeneProduct ensembl:ENSRNOG00000004864
Pskh1 GeneProduct ensembl:ENSRNOG00000019290
Snrpb2 GeneProduct ensembl:ENSRNOG00000004967
Hnrnph2 GeneProduct ensembl:ENSRNOG00000011661
Snrnp40 GeneProduct ensembl:ENSRNOG00000012219
Hnrnpu GeneProduct ensembl:ENSRNOG00000033790
PCBP2 GeneProduct None
Lsm7 GeneProduct ensembl:ENSRNOG00000019552
Ddx1 GeneProduct ensembl:ENSRNOG00000006652
Srpk1 GeneProduct ensembl:ENSRNOG00000000511
U2af1 GeneProduct ensembl:ENSRNOG00000045860
Tra2b GeneProduct ensembl:ENSRNOG00000001783
SRPK2 GeneProduct None
HNRPA3P1 GeneProduct None
Hnrnpk GeneProduct ensembl:ENSRNOG00000019113
Snu13 GeneProduct ensembl:ENSRNOG00000025154
Supt5h GeneProduct ensembl:ENSRNOG00000032034
Srp54a GeneProduct ensembl:ENSRNOG00000032776
Prpf4b GeneProduct ensembl:ENSRNOG00000016705
Celf1 GeneProduct ensembl:ENSRNOG00000010379
Srsf1 GeneProduct ensembl:ENSRNOG00000050323
Clp1 GeneProduct ensembl:ENSRNOG00000007416
Snrpf GeneProduct ensembl:ENSRNOG00000005556
Mettl3 GeneProduct ensembl:ENSRNOG00000013111
Srsf6 GeneProduct ensembl:ENSRNOG00000006380
Nxf1 GeneProduct ensembl:ENSRNOG00000019069
Sf3b2 GeneProduct ensembl:ENSRNOG00000020412
Sfpq GeneProduct ensembl:ENSRNOG00000058461
Rbm5 GeneProduct ensembl:ENSRNOG00000018153
Prpf3 GeneProduct ensembl:ENSRNOG00000025629
Snu13 GeneProduct ensembl:ENSRNOG00000025154
U5 snRNA Rna None
U1 snRNA Rna None
Srsf10 GeneProduct ensembl:ENSRNOG00000007992
Cstf3 GeneProduct ensembl:ENSRNOG00000012089
Papola GeneProduct ensembl:ENSRNOG00000004827
Hnrnpd GeneProduct ensembl:ENSRNOG00000002292
Cpsf3 GeneProduct ensembl:ENSRNOG00000052418
LOC103689931 GeneProduct ensembl:ENSRNOG00000003645
Clasrp GeneProduct ensembl:ENSRNOG00000046000
POLR2A GeneProduct None
Dicer1 GeneProduct ensembl:ENSRNOG00000010711
Pabpn1 GeneProduct ensembl:ENSRNOG00000042195
U2 GeneProduct ensembl:ENSRNOG00000061106
Prmt1 GeneProduct ensembl:ENSRNOG00000026109
Cpsf4 GeneProduct ensembl:ENSRNOG00000000985
Sfswap GeneProduct ensembl:ENSRNOG00000000931
Srsf3 GeneProduct ensembl:ENSRNOG00000000520
Sf3a1 GeneProduct ensembl:ENSRNOG00000005218
Cd2bp2 GeneProduct ensembl:ENSRNOG00000017201
AC126899.1 GeneProduct ensembl:ENSRNOG00000011065
Srsf10 GeneProduct ensembl:ENSRNOG00000007992
Tmed10 GeneProduct ensembl:ENSRNOG00000007901
Cpsf2 GeneProduct ensembl:ENSRNOG00000005733
Cpsf1 GeneProduct ensembl:ENSRNOG00000030705
Srsf2 GeneProduct ensembl:ENSRNOG00000000248
Ppm1g GeneProduct ensembl:ENSRNOG00000026905
Nudt21 GeneProduct ensembl:ENSRNOG00000042983
Sf3b4 GeneProduct ensembl:ENSRNOG00000021181
Cstf2 GeneProduct ensembl:ENSRNOG00000048025
Dhx38 GeneProduct ensembl:ENSRNOG00000014619
HNRPC GeneProduct None
Srek1 GeneProduct ensembl:ENSRNOG00000032735
Srsf4 GeneProduct ensembl:ENSRNOG00000010007
Clk2 GeneProduct ensembl:ENSRNOG00000020500
Prpf8 GeneProduct ensembl:ENSRNOG00000003495
Sf3a3 GeneProduct ensembl:ENSRNOG00000007629
GMP Metabolite cas:85-32-5
Snrpn GeneProduct ensembl:ENSRNOG00000059764
Clk4 GeneProduct ensembl:ENSRNOG00000003714
Sf3b1 GeneProduct ensembl:ENSRNOG00000013516
Prpf4 GeneProduct ensembl:ENSRNOG00000014777
Prmt2 GeneProduct ensembl:ENSRNOG00000001297
Ddx20 GeneProduct ensembl:ENSRNOG00000015274
Nono GeneProduct ensembl:ENSRNOG00000003689
Celf4 GeneProduct ensembl:ENSRNOG00000014503
Srsf5 GeneProduct ensembl:ENSRNOG00000005513
Prpf18 GeneProduct ensembl:ENSRNOG00000018396
Cstf2t GeneProduct ensembl:ENSRNOG00000050289
Ptbp1 GeneProduct ensembl:ENSRNOG00000010448
Snrpn GeneProduct ensembl:ENSRNOG00000059764
Cstf1 GeneProduct ensembl:ENSRNOG00000004775
AABR07051190.1 GeneProduct ensembl:ENSRNOG00000005062
U2af2 GeneProduct ensembl:ENSRNOG00000015914
ATP Metabolite cas:1927-31-7
Srsf7 GeneProduct ensembl:ENSRNOG00000027360
Dhx9 GeneProduct ensembl:ENSRNOG00000002735
Sugp2 GeneProduct ensembl:ENSRNOG00000020269
Srrm1 GeneProduct ensembl:ENSRNOG00000018194
Rnmt GeneProduct ensembl:ENSRNOG00000016698
Snrpep2 GeneProduct ensembl:ENSRNOG00000034206
Cdc40 GeneProduct ensembl:ENSRNOG00000000581
SNRPG GeneProduct None
Rbmx GeneProduct ensembl:ENSRNOG00000000866
Snrpb GeneProduct ensembl:ENSRNOG00000006961
Smc1a GeneProduct ensembl:ENSRNOG00000003139
Snrnp70 GeneProduct ensembl:ENSRNOG00000020763
Prpf8 GeneProduct ensembl:ENSRNOG00000003495
Txnl4a GeneProduct ensembl:ENSRNOG00000052004
Ncbp1 GeneProduct ensembl:ENSRNOG00000023605
Cdc40 GeneProduct ensembl:ENSRNOG00000000581
Dhx8 GeneProduct ensembl:ENSRNOG00000020772
Clk1 GeneProduct ensembl:ENSRNOG00000025768
Clk3 GeneProduct ensembl:ENSRNOG00000030126
Ptbp2 GeneProduct ensembl:ENSRNOG00000010827
Eftud2 GeneProduct ensembl:ENSRNOG00000002818
AC126899.1 GeneProduct ensembl:ENSRNOG00000011065
Rbm39 GeneProduct ensembl:ENSRNOG00000019848
Snrpa GeneProduct ensembl:ENSRNOG00000001501
Hnrnph1 GeneProduct ensembl:ENSRNOG00000003399
Fus GeneProduct ensembl:ENSRNOG00000023360
LOC688526 GeneProduct ensembl:ENSRNOG00000027239
Dhx15 GeneProduct ensembl:ENSRNOG00000003844

References

  1. Carmody SR, Wente SR. mRNA nuclear export at a glance. J Cell Sci. 2009 Jun 15;122(Pt 12):1933–7. PubMed Europe PMC Scholia